Marijuana Dip card
Urine based Marijuana Dip card screening tests for drugs of abuse range from simple immunoassay tests to complex analytical procedures. The speed and sensitivity of immunoassays have made them the most widely accepted method for screening urine for drugs of abuse.
The Marijuana Cassettes / Panels are based on the principle of the highly specific immunochemical reactions of antigens and antibodies, which are used for the analysis of specific compounds in human urine. The Marijuana Dip Cards are rapid, visual, competitive panel immunoassay that can be used for the simultaneous, qualitative detection of 11-nor- 9-tetrahydrocannabinol-9-carboxylic acid. The length of time following drug use for which a positive result may occur is dependent upon several factors including the frequency and amount of drug, metabolic rate, excretion rate, drug half-life, and the drug user’s age, weight, activity, and diet.
THC Dip Card Test Procedure:
- Remove the Marijuana Dip card from its sealed pouch, or remove from the canister, and use it as soon as possible. For the best results, the test should be performed within an hour. The canister should be closed tightly after removing any strips.
- Dip the sample pad area of the dipstick strip or dipstick card in the urine specimen submerging only
up to the “MAX” mark of the dipstick strip or the edge of the dipstick card.
- After the test has finished running, remove the strips from the specimen and place it on a non-absorbent flat surface. Start the timer and wait for the colored band(s) to appear. The result should be read for 5 minutes. Do not interpret the result after 10 minutes.
The Marijuana Dip card Cassettes / Panels are one-step immunoassays in which chemically labeled drugs (drug-protein conjugates) compete for limited antibody binding sites with drugs that may be present in urine. The test device contains membrane strips that are pre-coated with drug-protein conjugates on the test band(s). Each strip, the drug antibody-colloidal gold conjugate pad is placed at one end of the membrane.
In the absence of a drug in the urine, the solution of the colored antibody-colloidal gold conjugate moves along with the sample solution upward chromatographically by capillary action across the membrane to the immobilized drug-protein conjugate zone on the test band region. The colored antibody-gold conjugate then attaches to the drug-protein conjugates to form visible lines as the antibody complex with the drug conjugate. Therefore, the formation of the visible precipitant in the test zone occurs when the test urine is negative for the drug. When the drug is present in the urine, the drug/metabolite antigen competes with drug-protein conjugate on the test band region for the limited antibody. When a sufficient concentration of the drug is present, it will fill the limited antibody binding sites. This will prevent attachment of the colored antibody (drug-protein conjugate)-colloidal gold conjugate to the drug-protein conjugate zone on the test band region. Therefore, the absence of the color band on the test region indicates a positive result.
A control band with a different antigen/antibody reaction is added to the immunochromatographic membrane strip at the control region (C) to indicate that the test has performed properly. This control line should always appear regardless of the presence of drugs or metabolites. If the control line does not appear the test device should be discarded.
- Internal procedural controls are included in the test. A colored band appearing in the control region(C) is considered an internal positive procedural control, confirming sufficient specimen volume and correct procedural technique.
- External controls are not supplied with this kit. It is recommended that positive and negative controls be tested as a good laboratory practice to confirm the Marijuana Dip card test procedure to verify proper test performance.