For what is EtG/Alcohol Test used
Rapid EtG/Alcohol Test Dip card is an immunochromatographic assay for the qualitative determination of the presence of Ethyl glucuronide (ETG) at the cut off of 500 ng/ml. This assay provides only a preliminary analytical test result. Gas Chromatography/Mass spectrometry (GC/MS) is the preferred confirmatory method. Clinical consideration and professional judgment should be applied to any drug of an abuse test result, particularly when preliminary positive results are indicated.
What is EtG
Ethyl Glucuronide (EtG) is a direct metabolite of alcohol. Presence in urine may be used to detect recent alcohol intake, even after alcohol is no longer measurable. Traditional laboratory methods detect the actual alcohol in the body, which reflects current intake within the past few hours (depending on how much was consumed). The presence of EtG in urine is a definitive indicator that it can be detected in the urine for 3 to 4 days after drinking alcohol, even alcohol is eliminated from the body. Therefore, EtG is a more accurate indicator of the recent intake of alcohol than measuring for the presence of alcohol itself. The EtG test can aid in the diagnosis of drunk driving and alcoholism, which has an important significance in forensic identification and medical examination.
How EtG/Alcohol Test works
Rapid Ethyl glucuronide (ETG) Test Dip Card is a competitive immunoassay that is used to screen for the presence of
Ethyl glucuronide (ETG) in the urine. It is a chromatographic absorbent device in which, drugs within a urine sample, competitively combined to a limited number of drug monoclonal antibody (mouse) conjugate binding sites.
When the test is activated, the urine is absorbed into each test Dip card by capillary action, mixes with the respective drug monoclonal antibody conjugate, and flows across a pre-coated membrane.
- Internal procedural controls are included in the test. A colored band appearing in the control region(C) is considered an internal positive procedural control, confirming sufficient specimen volume and correct procedural technique.
- External controls are not supplied with this kit. It is recommended that positive and negative controls be tested as a good laboratory practice to confirm the EtG Test Screen procedure to verify proper test performance.
EtG/Alcohol Test Screen Procedure:
- Remove the EtG/Alcohol Test Screen from its sealed pouch, or remove from the canister, and use it as soon as possible. For the best results, the test should be performed within an hour.
- Hold the EtG/Alcohol Test by the end, where the product name is printed. To avoid contamination, do not touch the strip membrane.
- Holding the strip vertically, dip the test strip in the urine specimen for at least 10-15 seconds. Do not immerse past the maximum line (MAX) on the test strip.
- After the test has finished running, remove the strip from the specimen and place it on a non-absorbent flat surface. Start the timer and wait for the colored band(s) to appear. The result should be read 5 minutes. Do not interpret the result after 10 minutes.